quantikine elisa mouse immunoassay scd14  (R&D Systems)

Journal: iScience

Article Title: Dietary fiber reduces mortality from secondary sepsis in a murine model of Clostridioides difficile infection

doi: 10.1016/j.isci.2026.115258

Figure Lengend Snippet: Plasma markers of systemic inflammation and kidney injury in CDI mice across diets (A) PCoA of Canberra distances of mean-normalized sepsis/immune marker concentrations. Points are colored by diet, and shapes indicate whether mice were humanely euthanized due to clinical sickness or survived until the experimental endpoint. Vectors show the correlation of each measured factor with PC1 and PC2, with the vector length indicating the relative strength of the correlation. Only statistically significant vectors are shown (multiple regression with Benjamini-Hochberg multiple test correction, p.adj. < 0.05). (B) Plasma concentration of each marker that significantly differed between diets (blood urea nitrogen (BUN), and immune factors sCD14, CXCL1, IL-10, IL-1B, IL-6, and TNF-a) at sacrifice. Pairwise comparisons of concentrations between diets were calculated using Kruskal-Wallis and Dunn’s post hoc tests, with p -value corrections conducted via Benjamini and Hochberg. Boxplot lines (from top to bottom) depict the 75 th , 50 th (median), and 25 th percentiles, with lines extending from the top/bottom of the boxplot indicating the largest/smallest observation within ±1.5∗IQR (inter-quartile range). P-value significance (∗∗∗∗: p < 0.0001, ∗∗∗: p < 0.001, ∗∗: p < 0.01, and ∗: p < 0.05).

Article Snippet: Quantikine ELISA Mouse Immunoassay – sCD14 , RnD Systems , MC140.

Techniques: Clinical Proteomics, Marker, Plasmid Preparation, Concentration Assay

Journal: Human Genetics and Genomics Advances

Article Title: Aberrant N-glycosylation may be a therapeutic target in carriers of a common and highly pleiotropic variant in the manganese transporter ZIP8

doi: 10.1016/j.xhgg.2025.100517

Figure Lengend Snippet: Defects in bile acid homeostasis and intestinal permeability are ameliorated with oral GlcNAc supplementation in Zip8 +/393T and Zip8 393T/393T mice (A) Total fecal bile acid in Zip8 +/+ (WT), Zip8 +/393T (Het), and Zip8 393T/393T (HM) mice with and without GlcNAc supplementation. (B and C) (B) mRNA expression of Fgf15 in ileum and (C) Cyp7a1 in liver in Zip8 +/+ (WT), Zip8 +/393T (Het), and Zip8 393T/393T (HM) mice with and without GlcNAc supplementation. mRNA data interpolated from standard curve using four parameter logistic curve (GraphPad Prism 9). (D) Serum sCD14 concentration using mouse CD14 immunoassay kit (catalog no. MC140, R&D Systems). Data are represented as mean ± SEM. Statistical significance was determined by two-way ANOVA with multiple comparison testing. p value indicated by one asterisk (<0.05), two asterisks (<0.01), three asterisks (<0.001), or four asterisks (<0.0001). n = 5–6 male mice/genotype.

Article Snippet: Defects in bile acid homeostasis and intestinal permeability are ameliorated with oral GlcNAc supplementation in Zip8 +/393T and Zip8 393T/393T mice (A) Total fecal bile acid in Zip8 +/+ (WT), Zip8 +/393T (Het), and Zip8 393T/393T (HM) mice with and without GlcNAc supplementation. (B and C) (B) mRNA expression of Fgf15 in ileum and (C) Cyp7a1 in liver in Zip8 +/+ (WT), Zip8 +/393T (Het), and Zip8 393T/393T (HM) mice with and without GlcNAc supplementation. mRNA data interpolated from standard curve using four parameter logistic curve (GraphPad Prism 9). (D) Serum sCD14 concentration using mouse CD14 immunoassay kit (catalog no. MC140, R&D Systems).

Techniques: Permeability, Expressing, Concentration Assay, Comparison

Journal: Cell Death & Disease

Article Title: SMAC mimetic drives microglia phenotype and glioblastoma immune microenvironment

doi: 10.1038/s41419-024-07056-z

Figure Lengend Snippet: A Representative experiment of 3 independent experiments of IAP expression levels analyzed by Western blotting after 72 h of vehicle or SMg treatment in C8B4 microglia cell line. B Quantification of GL261-DsRed spheroids area upon vehicle ( n = 10) and SMg treatment ( n = 9) and in the presence or not of the C8B4 cells ( n = 10). Statistical analyses were performed by using ANOVA test and ANOVA post-hoc Tukey test; alpha = 0.05, bilateral p -value: *** p < 0.0005. C Concentrations of CD14 and CCL17/TARC quantified in the supernatant of C8B4 and GL261-DsRed co-cultures by ELISA assay after 72 h of vehicle or SMg treatments ( n = 6 independent experiments). Statistical analyses were performed by using Mann–Whitney test; alpha = 0.05, bilateral p-value: ** p < 0.005. D Quantification of GL261-DsRed spheroids area in the presence of the C8B4 cells expressing ML-IAP (siCTRL) or down-expressing ML-IAP (siML-IAP) ( n = 10) after 72 h of treatment vehicle ( n = 10) and SMg treatment ( n = 9). Data from three independent experiments. Statistical analyses were performed by using ANOVA test and ANOVA post-hoc Tukey test; alpha = 0.05, bilateral p -value: * p < 0.05 ; ** p < 0.005 ; **** p < 0.0001. E Quantification of GL261-DsRed spheroid area in the presence of C8B4 cells treated with vehicle and SMg for 72 h. C8B4 cells were pre-treated for 24 h with ZVAD (vehicle n = 23, SMg n = 23) and TNFαi (vehicle n = 27, SMg n = 23) or without pre-treatment (vehicle n = 28, SMg n = 25). Statistical analyses were performed by using ANOVA test and ANOVA post-hoc Tukey test; alpha = 0.05, bilateral p -value: *** p < 0.0005. F Representative experiment ( n = 2) of IAP expression levels analyzed by western blotting after 72 h of vehicle or SMg treatment in C8B4 microglia cell line. C8B4 cells were pre-treated for 24 h with ZVAD and TNFαi. G Representative experiment ( n = 2) of expression levels of p65, phospho-p65, IκBα, phospho IκB, caspase-3, cleaved caspase-3 analyzed by Western blotting in C8B4 microglia cell line after 24 h of vehicle or SMg treatment. H Representative experiment out of 3 experiments of expression levels of CD206 and iNOS analyzed by Western blotting after 24 h of vehicle or SMg treatment. C8B4 cells were pre-treated for 24 h with ZVAD and TNFαi. Expression level of actin β served as loading control. I iNOS/CD206 ratio in C8B4 microglia cell line after 24 h of vehicle or SMg treatment. C8B4 cells were pre-treated for 24 h with ZVAD and TNFαi. Quantification was performed from 3 independent experiments using ImageJ software and data presented were normalized to actin β expression. iNOS/CD206 ratio fold changes were normalized on vehicle condition. B – E , I Bar graphs represent mean ± s.e.m.

Article Snippet: Supernatants were collected after 72 h of treatment and processed using the proteome profiler human XL cytokine array kit (R&D systems, #ARY022B) or the ELISA kits Quantikine ELISA Mouse CCL17/TARC Immunoassay (R&D systems, MCC170) and Mouse CD14 Immunoassay (R&D systems, MC140) accordingly to the manufacturer protocol (Data represented were normalized to reference spot expression in array membrane).

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Control, Software